Bianca Corriea, visiting exchange-student researcher working in the Wyatt Lab, is using antisense RNA (asRNA) to verify the candidate gene responsible for the unique phenotype of the gps3 mutant in response to GPS treatment.
To test the gps3 candidate gene, Corriea must silence the expression of this single gene and verify that this targeted transformation results in the gps 3 phenotype. The gene silencing method that Corriea will deploy is to sequence the candidate gene and then splice a copy of the gene into agrobacterium. Agrobacterium is used in plant biology as a method of introducing genes into the genome of test plants through a process known as agrobacterium-mediated transformation mechanism. This mechanism takes advantage of the agrobacterias natural process of invading host organisms and semi-randomly inserting its DNA into the host genome.