Wyatt Lab graduate student researcher, Marilyn Hayden narrates the NASA BRIC-20 de-integration process during science verification testing (SVT) July 28, 2014 at Kennedy Space Center. Continue reading
The Wyatt Lab researchers studying the gps2 mutant genome have identified a gene that appears to play a significant role in plant signal transduction. The candidate gene was identified through deep sequencing of the gps2 mutant genome and then comparing the results to the wild type (WT) genome. The analysis revealed a difference of a single gene in one area of the genome associated with plant signaling which had been disrupted by a semi-random T-DNA insertion that silenced (or shut off) the gene in the mutant. In the WT genome, the gps2 gene is intact and expresses normally during GPS treatment. Continue reading
On July 8th, Dr. Wyatt and Dr. Luesse, principle investigators for the NASA BRIC-20 experiment, met at the Kennedy Space Center in Cape Canaveral, Florida to begin work on the science verification testing (SVT) phase of the project. Continue reading
Dr. Wyatt paused to answer a few impromptu questions about her research. Twelve minutes later she was out the door and heading for the Plant Biology 2014 conference and on to the Kennedy Space Center for BRIC-20 SVT part 2. Continue reading
Arabidopsis (common name) gps mutants filmed in time-lapse photography (60 minutes shown) after being returned to room temperature responding to gravity persistent signaling (GPS) treatment.
NASA engineers refer to the point where the science experiment materials are loaded into the NASA BRIC and PDFU hardware as “integrating the science.” Continue reading
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The Wyatt Lab grows the Arabidopsis plants it studies from seedlings. The images below show a generation of plants in the growth chamber. (1) A single inflorescence stem towers above the crowd. (2) The plants are tagged and labeled for sample identification. (3-4) … Continue reading
Upon their return to Earth, the space flown seedlings from the BRIC-20 experiment will undergo both RNA and proteomic analyses. The two analyses used together will pinpoint the genes involved in plant signaling in microgravity conditions. Continue reading
Before the BRIC-20 project could move into the SVT phase, Wyatt Lab graduate student researcher, Proma Basu, had to solve three problems introduced by the logistics of sending seeds to space to germinate and then returning them to the lab for analysis. Continue reading